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pGSM21
pGSM21
規(guī)格:
貨期:
編號:B228800
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pGSM21
商品貨號 B228800
Designations pGSM21
Species Homo sapiens, human
Vector
Construct size (kb): 13.30000019073486
Insert
DNA: genomic
Insert lengths(kb): 9.0
Gene product: DNA Segment, single copy, expressed [D21S13]
Alleles: A1, A2, E1, E2, A1, A2, E1, E2
Insert Size (kb) 9.0
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
D21S13 contains a CpG island. The insert contains SmaI (2), PvuII (4), BssHII (2), SacII (1), NotI (1), and CfoI (1) sites.
Restriction digests of the clone give the following sizes (kb): EcoRI--8.5, 4.0; HindIII--13.0; PstI--4.9, 2.4, 1.7, 1.35, 0.88, 0.68; BamHI--4.0 (doublet), 2.9, 0.88; SalI--13.0.
An 8.4 kb EcoRI fragment contains a methylation-free HTF (HpaII tiny fragment) island and the following restriction sites: ApaI (1), BssHII (2), SacII (2), NotI (1), XbaI (1), and KpnI (1).
Isolated from the chromosome 22 library of R. Krumlauf et al.
References

Stewart GD, et al. Trisomy 21 (Down syndrome): studying nondisjunction and meoitic recombination by using cytogenetic and molecular polymorphisms that span chromosome 21. Am. J. Hum. Genet. 42: 227-236, 1988. PubMed: 2893544

Owen MJ, et al. Physical mapping around the Alzheimer disease locus on the proximal long arm of chromosome 21. Am. J. Hum. Genet. 46: 316-322, 1990. PubMed: 2301399

Van Keuren ML, et al. Characterization of an unusual and complex chromosome 21 rearrangement using somatic cell genetics and cloned DNA probes. Am. J. Med. Genet. 33: 369-375, 1989. PubMed: 2529766

Leveillard T, et al. A high frequency EcoRI RFLP detected at the D21S13 locus. Nucleic Acids Res. 18: 1319, 1990. PubMed: 1969629

Stinissen P, et al. The pericentromeric 21 DNA marker pGSM21 (D21S13) contains an expressed HTF island. Genomics 7: 119-122, 1990. PubMed: 1692297

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