国产91亚洲福利精品一区二区,国产综合成人久久大片91,国产成人精品久久综合,久久久91精品国产一区二区三区,91福利国产在线在线播放,91精品国产高清久久久久久91,91精品国产福利在线观看麻豆,国产免费一区二区三区在线观看

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > p414 GALS
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • [email protected]
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
p414 GALS
p414 GALS
規(guī)格:
貨期:
編號:B228224
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 p414 GALS
商品貨號 B228224
Designations p414 GALS
Depositors M Funk
Biosafety Level 1
Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 5.4289999008178710
DESCRIPTION OF VECTOR:
Intact vector size: 5.429
Type of vector: plasmid
Cloning sites: SpeI BamHI SmaI PstI EcoRI ClaI SalI XhoI
Polylinker sites: XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI XhoI
Construction: pRS414, GALS promoter
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
marker(s): TRP1, ->, 200-1213
terminator: CYC1, ->, 1895-2155
MCS: XhoI...SpeI, ->, 2155-2212
promoter for expression: GALS, <-, 2219-2634
marker(s): ampR, <-, 3881-4811
centromere: CEN6/ARSH4, ->, 4915-5429
Vector: p414 GALS (plasmid)
Promoters: Promoter for expression GALS
Construction: pRS414, GALS promoter
Marker(s):TRP1,ampR
Construct size (kb): 5.428999900817871
Features: marker(s): TRP1
marker(s): ampR
promoter for expression: GALS
MCS: XhoI...SpeI
terminator: CYC1
centromere: CEN6/ARSH4
Applications
YX-type (expression) shuttle vector
vector with low copy number
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.4; EcoRI--5.4; SacI/XbaI--3.2, 1.8, 0.41.
The galactokinase (GALS) promoter is a deletion variant of GAL1, which lacks one and a half of the 3 UAS elements required for full induction of the promoter by galactose.
The promoter is tightly repressed by glucose and is induced at a moderate level by galactose.
Low copy number shuttle expression vector.
One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mumberg D, et al. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22: 5767-5768, 1994. PubMed: 7838736

Related Products
component of:ATCC 87670
Shipped freeze-dried
Shipping Information Distributed: freeze-dried
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
林州市| 天等县| 东平县| 宁强县| 峨山| 滨州市| 新宾| 集安市| 连城县| 崇明县| 东安县| 英山县| 青铜峡市| 长沙县| 昌吉市| 蕉岭县| 乌苏市| 韶关市| 呼玛县| 思茅市| 辽源市| 阿克| 泊头市| 广汉市| 宣汉县| 方正县| 永年县| 怀仁县| 石景山区| 龙海市| 盐山县| 衡阳县| 辽源市| 米易县| 松滋市| 新野县| 宾川县| 张家界市| 吴江市| 游戏| 皮山县|