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lambdaSP14
lambdaSP14
規(guī)格:
貨期:
編號:B227755
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 lambdaSP14
商品貨號 B227755
Designations lambdaSP14
Species Homo sapiens, human
Depositors M Lefranc
Vector
Construct size (kb): 44.70000076293945
Insert
DNA: genomic
Insert lengths(kb): 15.19999980926514
Tissue: T-lymphocyte SP-F7 cell line
Gene product: T cell receptor, gamma, variable region V3 [TCRGJP]
Alleles: A2, A1
Insert Size (kb) 15.200
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried bacteria-free lysate
Comments
Restriction digests of the clone give the following sizes (kb): SmaI--18.0, .5, 7.0, 6.2, 5.0; BamHI--20.0, 12.0, 9.0, 3.6; EcoRI--21.0, 12.0, 5.8, 2.3, 1.9, 1.3; XbaI--20.0, 15.0, 9.5;
PvuI--22.0, 15.0, 7.5; SacI--19.0 9.5, 6.5, 4.2, 4.0.
The insert contains the following restriction sites (approximate kb from the5' end): EcoRI--1.4, 3.2, 9.1, 10.7; 11.9; 13.8; HindIII--1.3, 5.8, 6.4, 8.3, 9.9, 12.1, 14.0; KpnI--3.4, 3.9, 8.1.
Contains a non-functional V9-JP rearrangement generating a frameshift in the N diversity region. Also contains germline V2 and an unusual reqrrangement abutting V3 to J1 recombination signals in an inverted orientation.
lambdaSP14 (ATCC 65836, 65837) and lambdaSP19 (ATCC 65838, 65839) are end clones from the same inversion (47 kb) event.
References

Alexandre D, et al. Reciprocal hybrid joints demonstrate successive V - J rearrangements on the same chromosome in the human TCR gamma locus. Int. Immunol. 3: 973-982, 1991. PubMed: 1836738

梅經(jīng)理 17280875617 1438578920
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于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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