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p5-1-25 [p1-25]
p5-1-25 [p1-25]
規(guī)格:
貨期:
編號:B227748
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 p5-1-25 [p1-25]
商品貨號 B227748
Designations p5-1-25 [p1-25]
Species Homo sapiens, human
Applications
The most efficient detection of RFLPs at the D2S3 locus is by use of the cosmid 1-5 subclones p5-1-25 (ATCC 59684) and p5-2-96 (ATCC 59000).
Vector
Construct size (kb): 8.600000381469727
Insert
DNA: genomic
Insert lengths(kb): 3.200000047683716
Gene product: DNA Segment, single copy [D2S3]
Alleles: C4, J10, A1, B1, B2, C1, C2, C3, D2, G4, J1, J3, J4, J5, J6, J7, J8, K2, A2, D1, E1, E2, G1, G2, G3, J2, J9, K1
Insert Size (kb) 3.200
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.4, 3.2; HindIII--8.6; EcoRI/HindIII--5.4, 3.2; PstI--6.0, 1.8, 0.88.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
p5-1-25 is a single copy subclone from cosmid 1-5.
The most efficient detection of RFLPs at the D2S3 locus is by use of the cosmid 1-5 subclones p5-1-25 (ATCC 59684) and p5-2-96 (ATCC 59000). Use of p5-1-30 (ATCC 59006) and p5-1-32 (ATCC 59002) probably adds little to the PIC.
Subclone 5-1-32 detects the PstI insert/deletion RFLP with TaqI but bands are larger and difficult to resolve.
Long runs on 20 cm long 1% agarose gels, such that all fragments less than about 1.5 kb have run off the end, are required to obtain adequate resolution.
References

Litt M. A single-copy subclone, p1-25, from cosmid 1-5, defines a highly polymorphic RFLP on 2q35->37 [HGM8 no. D2S3]. Nucleic Acids Res. 14: 4378, 1986. PubMed: 3714483

Litt M, et al. A highly polymorphic locus in human DNA revealed by probes from cosmid 1-5 maps to chromosome 2q35-37. Am. J. Hum. Genet. 38: 288-296, 1986. PubMed: 3006480

Lathrop GM, et al. Three genetic linkage groups on chromosome 2. Cytogenet. Cell Genet. 46: 644, 1987.

Asher JH Jr., et al. Waardenburg syndrome (WS): the analysis of a single family with a WS1 mutation showing linkage to RFLP markers on human chromosome 2q. Am. J. Hum. Genet. 48: 43-52, 1991. PubMed: 1670751

Litt M, White RL. A highly polymorphic locus in human DNA revealed by cosmid-derived probes. Proc. Natl. Acad. Sci. USA 82: 6206-6210, 1985. PubMed: 2994065

Michael Litt, personal communication

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