国产91亚洲福利精品一区二区,国产综合成人久久大片91,国产成人精品久久综合,久久久91精品国产一区二区三区,91福利国产在线在线播放,91精品国产高清久久久久久91,91精品国产福利在线观看麻豆,国产免费一区二区三区在线观看

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > pKR1B-LAC4-1 [L1582/pKR1B-LAC4-1, YNN27/pKR1B-LAC4-1]
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • [email protected]
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
pKR1B-LAC4-1 [L1582/pKR1B-LAC4-1, YNN27/pKR1B-LAC4-1]
pKR1B-LAC4-1 [L1582/pKR1B-LAC4-1, YNN27/pKR1B-LAC4-1]
規(guī)格:
貨期:
編號:B226911
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pKR1B-LAC4-1 [L1582/pKR1B-LAC4-1, YNN27/pKR1B-LAC4-1]
商品貨號 B226911
Designations pKR1B-LAC4-1 [L1582/pKR1B-LAC4-1, YNN27/pKR1B-LAC4-1]
GenBank Definition

Yeast (K.lactis) beta-galactosidase gene, 5' end

GenBank Number

M18108

Species Kluyveromyces lactis (Dombrowski) van der Walt
Depositors University of Kentucky, RC Dickson, University of Kentucky
Applications
produces protein beta-galactosidase beta-D-galactosidase
produces protein lactose permease
This is a Lac+ strain expressing K. lactis lactose permease and beta-galactosidase.
Vector
Construct size (kb): 20.0
Insert
DNA: genomic
Insert lengths(kb): 12.0
Gene product: beta-galactosidase(beta-D-galactosidase) [LAC4]
Target Gene: lactose permease, beta-galactosidase
Insert Size (kb) 12.0
Genotype MATalpha ino HIS4::lacZ
Media ATCC® Medium 1245: YEPD
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
The plasmid contains the following restriction sites (approximate kb from the pBR322-derived EcoRI site): XhoI--2.5; EcoRI--8.1, 9.1, 11.4, 11.9, 12.6, 15.1, 16; BamHI--14.6, 19.7.
Fermentation of lactose in whey
GenBank M18108 contains the sequence for the promoter region and part of the coding sequence for LAC4.
The vector is a derivative of pBR322 carrying the kanamycin (G418) resistance gene of Tn903 and ARS1B from Kluyveromyces lactis. This plasmid can replicate in E. coli, S. cerevisiae, and K. lactis.
The order of the major features in this plasmid is: ampR - kanR - LAC12 - LAC4 > - ARS1B.
References

Sreekrishna K, et al. Transformation of Kluyveromyces lactis with the kanamycin (G418) resistance gene of Tn903. Gene 28: 73-81, 1984. PubMed: 6329916

Sreekrishna K, Dickson RC. Construction of strains of Saccharomyces cerevisiae that grow on lactose. Proc. Natl. Acad. Sci. USA 82: 7909-7913, 1985. PubMed: 3934664

Leonardo JM, et al. Identification of upstream activator sequences that regulate induction of the beta-galactosidase gene in Kluyveromyces lactis. Mol. Cell. Biol. 7: 4369-4376, 1987. PubMed: 3125422

Dickson RC, Sreekrishna KK. Lac+ Saccharomyces cerevisiae. US Patent 5,047,340 dated Sep 10 1991

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
龙南县| 旬阳县| 通江县| 井研县| 伊金霍洛旗| 元谋县| 南汇区| 韩城市| 会宁县| 泸州市| 连平县| 石屏县| 方正县| 宝清县| 清徐县| 喀什市| 桐庐县| 绍兴市| 清河县| 苗栗市| 布尔津县| 邻水| 绥阳县| 日喀则市| 郑州市| 涟源市| 龙山县| 巴青县| 赣州市| 滦平县| 夏津县| 弋阳县| 稷山县| 光山县| 新兴县| 霍林郭勒市| 双辽市| 自贡市| 仪陇县| 陵川县| 怀宁县|