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pKR1B-LAC4-1 [L1582/pKR1B-LAC4-1, YNN27/pKR1B-LAC4-1]
pKR1B-LAC4-1 [L1582/pKR1B-LAC4-1, YNN27/pKR1B-LAC4-1]
規(guī)格:
貨期:
編號:B226911
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pKR1B-LAC4-1 [L1582/pKR1B-LAC4-1, YNN27/pKR1B-LAC4-1]
商品貨號 B226911
Designations pKR1B-LAC4-1 [L1582/pKR1B-LAC4-1, YNN27/pKR1B-LAC4-1]
GenBank Definition

Yeast (K.lactis) beta-galactosidase gene, 5' end

GenBank Number

M18108

Species Kluyveromyces lactis (Dombrowski) van der Walt
Depositors University of Kentucky, RC Dickson, University of Kentucky
Applications
produces protein beta-galactosidase beta-D-galactosidase
produces protein lactose permease
This is a Lac+ strain expressing K. lactis lactose permease and beta-galactosidase.
Vector
Construct size (kb): 20.0
Insert
DNA: genomic
Insert lengths(kb): 12.0
Gene product: beta-galactosidase(beta-D-galactosidase) [LAC4]
Target Gene: lactose permease, beta-galactosidase
Insert Size (kb) 12.0
Genotype MATalpha ino HIS4::lacZ
Media ATCC® Medium 1245: YEPD
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
The plasmid contains the following restriction sites (approximate kb from the pBR322-derived EcoRI site): XhoI--2.5; EcoRI--8.1, 9.1, 11.4, 11.9, 12.6, 15.1, 16; BamHI--14.6, 19.7.
Fermentation of lactose in whey
GenBank M18108 contains the sequence for the promoter region and part of the coding sequence for LAC4.
The vector is a derivative of pBR322 carrying the kanamycin (G418) resistance gene of Tn903 and ARS1B from Kluyveromyces lactis. This plasmid can replicate in E. coli, S. cerevisiae, and K. lactis.
The order of the major features in this plasmid is: ampR - kanR - LAC12 - LAC4 > - ARS1B.
References

Sreekrishna K, et al. Transformation of Kluyveromyces lactis with the kanamycin (G418) resistance gene of Tn903. Gene 28: 73-81, 1984. PubMed: 6329916

Sreekrishna K, Dickson RC. Construction of strains of Saccharomyces cerevisiae that grow on lactose. Proc. Natl. Acad. Sci. USA 82: 7909-7913, 1985. PubMed: 3934664

Leonardo JM, et al. Identification of upstream activator sequences that regulate induction of the beta-galactosidase gene in Kluyveromyces lactis. Mol. Cell. Biol. 7: 4369-4376, 1987. PubMed: 3125422

Dickson RC, Sreekrishna KK. Lac+ Saccharomyces cerevisiae. US Patent 5,047,340 dated Sep 10 1991

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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