国产91亚洲福利精品一区二区,国产综合成人久久大片91,国产成人精品久久综合,久久久91精品国产一区二区三区,91福利国产在线在线播放,91精品国产高清久久久久久91,91精品国产福利在线观看麻豆,国产免费一区二区三区在线观看

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > Hs 856.T
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
Hs 856.T
Hs 856.T
規(guī)格:
貨期:
編號:B164752
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Hs 856.T
商品貨號 B164752
Organism Homo sapiens, human
Tissue connective tissue
Product Format frozen
Morphology mixed
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease histiocytoma
Age 16 years
Gender female
Ethnicity Black
Karyotype modal number = 46; range = 45 to 46
Images ATCC CRL-7593 Cell Micrograph
Clinical Data
female
Black
16 years
Comments
Part of the NBL Collection. Unlike other cell lines in the NBL Collection, this item has been fully accessioned by ATCC and is covered by the standard warranty.
Please see the NBL Repository description.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  • Remove and discard culture medium.
  • Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  • Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
  • Add 2.0 to 3.0 ml of complete growth medium and aspirate cells by gently pipetting
  • Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
  • Incubate cultures at 37C.
Subcultivation ratio: A subcultivation ratio of 1:3 to 1:6 is recommended.
Medium renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X
CSF1PO: 7, 8
D13S317: 11
D16S539: 11, 12
D5S818: 8, 11
D7S820: 11
THO1: 7, 9
TPOX: 7, 10
vWA: 15, 18
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
徐州市| 克拉玛依市| 杨浦区| 平和县| 丽江市| 华容县| 黄陵县| 虹口区| 咸宁市| 潜江市| 桃园市| 克什克腾旗| 东阿县| 集贤县| 晋宁县| 阿克| 蒲江县| 安塞县| 康马县| 泸水县| 六盘水市| 庆城县| 舞阳县| 遵化市| 砀山县| 武乡县| 孝义市| 莲花县| 马公市| 剑川县| 运城市| 沐川县| 团风县| 绥江县| 鹿泉市| 原平市| 安西县| 陇川县| 安宁市| 翁源县| 新巴尔虎左旗|