| 產(chǎn)品名稱 |
HaK |
| 商品貨號 |
B164548 |
| Organism |
Mesocricetus auratus, hamster, Syrian golden |
| Tissue |
kidney |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
normal |
| Age |
adult |
| Gender |
male |
| Storage Conditions |
liquid nitrogen vapor phase |
| Tumorigenic |
Yes |
| Effects |
Yes, in hamsters (Tumors developed in 5/9 hamsters injected intra-cerebrally with 0.03 ml containing 7000 to 15000 cells (Moore, AE, personal communication).) |
| Virus Resistance |
poliovirus 1, 2, 3; adenovirus 3; coxsackievirus A4, A8, B1; herpes simplex; smallpox (variola); influenzaviruses (Asian) |
| Complete Growth Medium |
Complete Growth Medium
The base medium for this cell line is Eagle's Minimum Essential Medium (ATCC® No. 30-2003).
To make the complete growth medium, add the following components to the base medium:
Calf Serum (ATCC® No. 30-2030) to a final concentration of 10%.
This medium is formulated for use in 5% CO2. |
| Subculturing |
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
- Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37ºC to facilitate dispersal.
- Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation |
Complete growth medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions |
Temperature: 37°C
Atmosphere: 5% CO2
|
| Name of Depositor |
AE Moore |
| Deposited As |
Mesocricetus auratus |
